|
Intergenic (crossover) recombination in rec12 mutants. |
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| Genetic interval (chromosome) |
No. testeda |
No. recombinanta |
Recombinant freq. (%) |
cMb |
cM in wild-typec |
Fold reductionc |
|
|
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| A. rec12-D15 |
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|
|
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| lys1 – his1 (I) |
500 |
3 |
0.6 |
0.6 |
89 |
148 |
| lys1 – ade4 (I) |
500 |
8 |
1.6 |
1.6 |
284 |
177 |
| his1 – ade2 (I) |
500 |
2 |
0.4 |
0.4 |
57 |
142 |
| his1 – tps19 (I) |
500 |
2 |
0.4 |
0.4 |
143 |
357 |
| ade2 – tps19 (I) |
500 |
0 |
≤ 0.2 |
≤ 0.2 |
86 |
≤ 430 |
| his4 – ade1 (II) |
500 |
6 |
1.2 |
1.2 |
63 |
53 |
| ade6 – arg1 (III) |
500 |
1 |
0.2 |
0.2 |
97 |
486 |
| mean = |
256 ± 154 |
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|
|
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| B. rec12-Y98F |
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|
|
||||||
| lys1 – his1 (I) |
500 |
3 |
0.6 |
0.6 |
89 |
148 |
| lys1 – ade4 (I) |
500 |
17 |
3.4 |
3.5 |
284 |
81 |
| his1 – ade2 (I) |
500 |
1 |
0.2 |
0.2 |
57 |
285 |
| his1 – tps19 (I) |
500 |
1 |
0.2 |
0.2 |
143 |
715 |
| ade2 – tps19 (I) |
500 |
0 |
≤ 0.2 |
≤ 0.2 |
86 |
≤ 430 |
| his4 – ade1 (II) |
500 |
5 |
1.0 |
1.0 |
63 |
63 |
| ade6 – arg1 (III) |
500 |
4 |
0.8 |
0.8 |
97 |
121 |
| mean = |
263 ± 220 |
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|
aSpore colonies were replica plated on non-selective and selective media; the number of colonies recombinant for each genetic interval are indicated. Diploid (potentially complementing) spore colonies were excluded from analysis. bGenetic distance calculated using the mapping function of Haldane [66]. cFold reduction in crossing over relative to published map distances in wild-type cells (NCBI Entrez Genome) [36]. The strains crossed were: WSP1826 × WSP1967; WSP1825 × WSP1962; WSP1967 × WSP1973; WSP1967 × WSP1973; WSP1967 × WSP1973; WSP2015 × WSP2018; WSP2026 × WSP2024; WSP2020 × WSP1966; WSP1670 × WSP1968; WSP1966 × WSP2027; WSP1966 × WSP2027; WSP1966 × WSP2027; WSP2014 × WSP2011; WSP1809 × WSP2028. Strain genotypes are provided in Table 3. | ||||||
Sharif et al. Cell & Chromosome 2002 1:1 doi:10.1186/1475-9268-1-1 |
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