Review
Imaging genome abnormalities in cancer research
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* Corresponding author: Henry HQ Heng hheng@genetics.wayne.edu
1 Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA
2 Department of Pathology, Wayne State University School of Medicine, Detroit, MI, USA
3 Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI, USA
4 SeeDNA Biotech Inc, Windsor, Ontario, Canada
Cell & Chromosome 2004, 3:1 doi:10.1186/1475-9268-3-1
Published: 13 January 2004Abstract
Increasing attention is focusing on chromosomal and genome structure in cancer research due to the fact that genomic instability plays a principal role in cancer initiation, progression and response to chemotherapeutic agents. The integrity of the genome (including structural, behavioral and functional aspects) of normal and cancer cells can be monitored with direct visualization by using a variety of cutting edge molecular cytogenetic technologies that are now available in the field of cancer research. Examples are presented in this review by grouping these methodologies into four categories visualizing different yet closely related major levels of genome structures. An integrated discussion is also presented on several ongoing projects involving the illustration of mitotic and meiotic chromatin loops; the identification of defective mitotic figures (DMF), a new type of chromosomal aberration capable of monitoring condensation defects in cancer; the establishment of a method that uses Non-Clonal Chromosomal Aberrations (NCCAs) as an index to monitor genomic instability; and the characterization of apoptosis related chromosomal fragmentations caused by drug treatments.