Calyculin A, an enhancer of myosin, speeds up anaphase chromosome movement

Additional file 1:

Movie representing meiosis I in control crane-fly spermatocyte. Two half-bivalent pairs (out of the total three) are in focus. After the autosomes reached the poles, the two smaller univalents sex chromosomes, which stayed at the metaphase plate, start their anaphase, at about the same time as cytokinesis starts. This movie displays time-lapsed images, obtained from real-time sequences recorded on DVD, played back at 120× the recorded speed.

Format: MPG Size: 2.3MB Download file

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Additional file 2:

Anaphase in a crane-fly spermatocyte treated with 50 nM Calyculin A at 13:03:30. Two half-bivalent pairs are in focus. Autosomes accelerated their poleward movement after Calyculin A addition. After they reached the poles, they moved backwards, toward the equator, where they joined their partners from the opposite pole and the two univalent sex chromosomes. Cell does not enter cytokinesis. This movie displays time-lapsed images, obtained from real-time sequences recorded on DVD, played back at 120× the recorded speed.

Format: MPG Size: 1.8MB Download file

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Additional file 3:

Prometaphase in a crane-fly spermatocyte treated with 50 nM Calyculin A at 13:41:00. Two autosomal bivalents are in focus. After Calyculin A addition, the chromosomes started to rotate and to move randomly in the spindle, much faster than before Calyculin A. The chromosomes do not align at the metaphase plate and the cell does not enter anaphase. This movie displays time-lapsed images, obtained from real-time sequences recorded on DVD, played back at 120× the recorded speed.

Format: MPG Size: 1.7MB Download file

Playing the movie within this page requires QuickTime 6.4 or later and JavaScript. Read more